Hotspot

trnD-GUC-trnY-GUA

LSC · 29478-29593 · 116 bp

Back to Nepeta

Pi 0.0277
MarkerSeek score 72.20
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
116
variable_sites
10
pi
0.0277
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGTGCTCTAACCGATTGAACT TGGCAATATGTCTACGCTGG 47.6 50.0 58.0 57.8 179-185 bp 1.0 77.0
trnD-GUC-trnY-GUA_p2 GGTGCTCTAACCGATTGAACT GGCAATATGTCTACGCTGGT 47.6 50.0 58.0 57.8 178-184 bp 1.0 77.0
trnD-GUC-trnY-GUA_p3 GGTGCTCTAACCGATTGAACT ATGGATTCTTCCTGGGTCGA 47.6 50.0 58.0 58.1 236-242 bp 1.0 76.2
trnD-GUC-trnY-GUA_p4 GGTGCTCTAACCGATTGAACT TGGATTCTTCCTGGGTCGAT 47.6 50.0 58.0 58.1 235-241 bp 1.0 76.2
trnD-GUC-trnY-GUA_p5 GGTGCTCTAACCGATTGAACT GGATTCTTCCTGGGTCGATG 47.6 55.0 58.0 57.8 234-240 bp 1.0 75.3