Hotspot

trnS-GCU-trnR-UCU

LSC · 8258-9934 · 1677 bp

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Pi 0.0212
MarkerSeek score 72.50
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1677
variable_sites
106
pi
0.0212
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1728-1753 bp 1.0 71.5
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1727-1752 bp 1.0 71.5
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1734-1759 bp 1.0 70.9
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1733-1758 bp 1.0 70.8
trnS-GCU-trnR-UCU_p5 ACGGAAAGAGAGGGATTCGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1782-1807 bp 1.0 70.1