Hotspot

psbI-trnS-GCU

LSC · 8004-8169 · 166 bp

Back to Nepeta

Pi 0.0827
MarkerSeek score 77.20
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
166
variable_sites
31
pi
0.0827
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 GCGTAATCCTGGACGTGAAG TGGACTAAAGCGTCGGATTG 55.0 50.0 58.7 58.0 247-270 bp 1.0 86.2
psbI-trnS-GCU_p2 GCGTAATCCTGGACGTGAAG GTGGACTAAAGCGTCGGATT 55.0 50.0 58.7 58.0 248-271 bp 1.0 86.2
psbI-trnS-GCU_p3 GCGTAATCCTGGACGTGAAG ATTGGGAGAGATGGCTGAGT 55.0 50.0 58.7 58.1 266-289 bp 1.0 85.4
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 246-269 bp 1.0 84.5
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 247-270 bp 1.0 84.5