Hotspot

psbK-psbI

LSC · 7508-7892 · 385 bp

Back to Nepeta

Pi 0.026
MarkerSeek score 68.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
385
variable_sites
29
pi
0.026
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TAGCTTTTGTTTGGCAGGCT ACGAAGAGTTTCAGAGTAAGCA 45.0 40.9 58.3 57.4 434-449 bp 1.0 65.1
psbK-psbI_p2 GCAGGCTGCTGTAAGTTTTC ACGAAGAGTTTCAGAGTAAGCA 50.0 40.9 57.7 57.4 421-436 bp 1.0 65.0
psbK-psbI_p3 AGGCTGCTGTAAGTTTTCGA ACGAAGAGTTTCAGAGTAAGCA 45.0 40.9 57.5 57.4 419-434 bp 1.0 63.4
psbK-psbI_p4 TTAGCTTTTGTTTGGCAGGC ACGAAGAGTTTCAGAGTAAGCA 45.0 40.9 57.5 57.4 435-450 bp 1.0 63.2
psbK-psbI_p5 CAGGCTGCTGTAAGTTTTCG ACGAAGAGTTTCAGAGTAAGCA 50.0 40.9 57.4 57.4 420-435 bp 1.0 62.9