Hotspot

ycf1

SSC · 151639-156914 · 5276 bp

Back to Nepenthes

Pi 0.014

MarkerSeek score 51.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5276
variable_sites: 74
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCATTTCGCCATTTTTGGGG	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.3	58.0	1094 bp	1.0	79.3
ycf1_p2	CATTTCGCCATTTTTGGGGT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.5	58.0	1093 bp	1.0	77.6
ycf1_p3	CCATTTCGCCATTTTTGGGG	CCCGAAAACGACCTTCCTTT	50.0	50.0	58.3	58.4	1081 bp	1.0	76.2
ycf1_p4	CCATTTCGCCATTTTTGGGG	CTTCCTTTTGGTTCTCCCCG	50.0	55.0	58.3	58.5	1097 bp	1.0	75.6
ycf1_p5	TCCATTTCGCCATTTTTGGG	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.2	58.0	1095 bp	1.0	75.3