Hotspot

rps19

IRb · 24995-25291 · 297 bp

Back to Nepenthes

Pi 0.0102

MarkerSeek score 35.60

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 297
variable_sites: 1
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.33
markerseek_score: 35.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGGGTAGAGCCGGATCTAAG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.0	58.0	1271 bp	0.5	59.6
rps19_p2	TTATTCGTCGCCGTAGCAAA	TCAGTGCTATGCATGGTTCC	45.0	50.0	58.0	58.0	1076 bp	0.5	59.6
rps19_p3	TGGGTAGAGCCGGATCTAAG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.0	58.0	1270 bp	0.5	59.6
rps19_p4	TTATTCGTCGCCGTAGCAAA	CAGTGCTATGCATGGTTCCT	45.0	50.0	58.0	58.0	1075 bp	0.5	59.6
rps19_p5	TGGGTAGAGCCGGATCTAAG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.0	57.9	888 bp	0.5	59.4