Hotspot

rpl32-trnL-UAG

SSC · 141066-142164 · 1099 bp

Back to Nepenthes

Pi 0.0219

MarkerSeek score 58.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1099
variable_sites: 24
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTAGGGTGGGCTGAGTTCT	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	2285-2292 bp	1.0	81.2
rpl32-trnL-UAG_p2	ATTAGGGTGGGCTGAGTTCT	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	2284-2291 bp	1.0	81.2
rpl32-trnL-UAG_p3	ATTAGGGTGGGCTGAGTTCT	TGCCCCGAATAAATCCAAGG	50.0	50.0	58.0	57.9	2013-2020 bp	1.0	81.2
rpl32-trnL-UAG_p4	GAAAGGGATATTGGGCAGCA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	57.9	58.0	1857-1861 bp	1.0	80.8
rpl32-trnL-UAG_p5	GAAAGGGATATTGGGCAGCA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	57.9	58.0	1856-1860 bp	1.0	80.8