Hotspot

rpl22-rps19

LSC · 112309-112350 · 42 bp

Back to Nepenthes

Pi 0.0476

MarkerSeek score 66.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 42
variable_sites: 2
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ATACGTGCAATTCCCTCGAC	TTATTCGTCGCCGTAGCAAA	50.0	45.0	58.1	58.0	1027 bp	1.0	80.4
rpl22-rps19_p2	ATACGTGCAATTCCCTCGAC	AAGCTTAACACAAAGGCGGA	50.0	45.0	58.1	58.0	889 bp	1.0	80.3
rpl22-rps19_p3	ATACGTGCAATTCCCTCGAC	AGCTTAACACAAAGGCGGAA	50.0	45.0	58.1	58.0	888 bp	1.0	80.3
rpl22-rps19_p4	ATACGTGCAATTCCCTCGAC	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1222 bp	1.0	80.2
rpl22-rps19_p5	ATACGTGCAATTCCCTCGAC	TCCCGGGCATCTACCATTAT	50.0	50.0	58.1	58.0	847 bp	1.0	80.2