Hotspot

rps3-rpl22

LSC · 111869-111879 · 11 bp

Back to Nepenthes

Pi 0.0909

MarkerSeek score 72.70

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 11
variable_sites: 1
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TCAGCCTGCTCGGTTAATTC	AAGCTTAACACAAAGGCGGA	50.0	45.0	58.0	58.0	1168 bp	1.0	80.4
rps3-rpl22_p2	TCAGCCTGCTCGGTTAATTC	AGCTTAACACAAAGGCGGAA	50.0	45.0	58.0	58.0	1167 bp	1.0	80.4
rps3-rpl22_p3	TCAGCCTGCTCGGTTAATTC	AATTGGGGGAATTTGTGCCT	50.0	45.0	58.0	58.0	1020 bp	1.0	80.4
rps3-rpl22_p4	TCAGCCTGCTCGGTTAATTC	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	1126 bp	1.0	80.3
rps3-rpl22_p5	ATACGTGCAATTCCCTCGAC	AGCTTAACACAAAGGCGGAA	50.0	45.0	58.1	58.0	888 bp	1.0	80.3