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psbT-psbN

LSC · 102665-102730 · 66 bp

Back to Nepenthes

Pi 0.0455

MarkerSeek score 71.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 3
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	180 bp	1.0	67.9
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	132 bp	1.0	67.2
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	57.6	57.4	155 bp	1.0	65.9
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	133 bp	1.0	65.9
psbT-psbN_p5	TCGAGAACCACCTAAAGTTCC	GGGTACGCCTTATATACCGC	47.6	55.0	57.3	57.7	181 bp	1.0	65.7