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psbE-petL

LSC · 92561-93831 · 1271 bp

Back to Nepenthes

Pi 0.0245

MarkerSeek score 65.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1271
variable_sites: 31
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1474-1477 bp	1.0	74.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1463-1466 bp	1.0	74.0
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1386-1389 bp	1.0	73.3
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1381-1384 bp	1.0	73.0
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1465-1468 bp	1.0	72.5