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petA-psbJ

LSC · 90729-91774 · 1046 bp

Back to Nepenthes

Pi 0.0172

MarkerSeek score 63.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 18
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	1174-1208 bp	1.0	77.9
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.7	57.6	1181-1215 bp	1.0	72.8
petA-psbJ_p3	GCCTTTTGTTCTTCTTCGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.2	57.6	1173-1207 bp	1.0	72.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	TGGTTCATATTCCGGATTGGG	50.0	47.6	57.9	57.8	1182-1216 bp	1.0	71.0
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	GGTTCATATTCCGGATTGGGT	50.0	47.6	57.9	57.8	1181-1215 bp	1.0	71.0