Hotspot

trnF-GAA-ndhJ

LSC · 75373-75887 · 515 bp

Back to Nepenthes

Pi 0.0117

MarkerSeek score 63.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 515
variable_sites: 6
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	652 bp	1.0	78.9
trnF-GAA-ndhJ_p2	ATATGATGCGTCGGGAATGG	TCCACGCCTGAAACGTATTT	50.0	45.0	57.9	57.8	710 bp	1.0	78.5
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	TCCACGCCTGAAACGTATTT	50.0	45.0	58.2	57.8	695 bp	1.0	77.7
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	TCCACGCCTGAAACGTATTT	50.0	45.0	58.2	57.8	694 bp	1.0	77.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	654 bp	1.0	77.5