Hotspot

trnT-UGU-trnL-UAA

LSC · 73303-74348 · 1046 bp

Back to Nepenthes

Pi 0.0154

MarkerSeek score 65.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 16
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	1119-1131 bp	1.0	79.9
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	GCGTCTACCATTTCGCCATA	55.0	50.0	57.9	58.1	1102-1114 bp	1.0	79.3
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	50.0	50.0	57.8	58.1	1151-1163 bp	1.0	79.0
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AAGTCCGTAGCGTCTACCAT	55.0	50.0	57.9	58.2	1111-1123 bp	1.0	78.5
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCATT	55.0	50.0	57.9	58.2	1110-1122 bp	1.0	78.5