Hotspot

psbZ-trnG-GCC

LSC · 62463-62756 · 294 bp

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Pi 0.171

MarkerSeek score 80.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 294
variable_sites: 49
pi: 0.171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	447-451 bp	1.0	86.2
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	445-449 bp	1.0	85.8
psbZ-trnG-GCC_p3	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	441-445 bp	1.0	81.0
psbZ-trnG-GCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	446-450 bp	1.0	80.4
psbZ-trnG-GCC_p5	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	442-446 bp	1.0	78.6