Hotspot

psbC-trnS-UGA

LSC · 61661-61851 · 191 bp

Back to Nepenthes

Pi 0.0681

MarkerSeek score 76.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 13
pi: 0.0681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCGATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.3	58.0	314-343 bp	1.0	80.9
psbC-trnS-UGA_p2	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	394-423 bp	1.0	80.8
psbC-trnS-UGA_p3	TCGTAGGTCATTTGTGGCAC	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.2	58.1	386-415 bp	1.0	80.6
psbC-trnS-UGA_p4	TCGTAGGTCATTTGTGGCAC	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.2	58.1	387-416 bp	1.0	80.6
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	379-408 bp	1.0	80.6