Hotspot

trnK-rps16

LSC · 29408-30116 · 709 bp

Back to Nepenthes

Pi 0.0161

MarkerSeek score 70.90

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 11
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAACTCTGCTGATGGTGTGG	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.1	58.2	893-902 bp	1.0	81.0
trnK-rps16_p2	TGGTGTGGACGAATCCTTTG	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.1	58.2	881-890 bp	1.0	80.9
trnK-rps16_p3	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	824-833 bp	1.0	80.7
trnK-rps16_p4	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	823-832 bp	1.0	80.7
trnK-rps16_p5	TGTGGACGAATCCTTTGCTT	GGTGCTCAACCTACAGGAAC	45.0	55.0	57.7	58.2	878-887 bp	1.0	79.7