Hotspot

trnH-GUG-psbA

LSC · 25268-25561 · 294 bp

Back to Nepenthes

Pi 0.0397

MarkerSeek score 66.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 294
variable_sites: 11
pi: 0.0397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TGGGTAGAGCCGGATCTAAG	CTGTTGCGTATTCAGCTCCT	55.0	50.0	58.0	58.0	1472 bp	0.5	59.8
trnH-GUG-psbA_p2	TTATTCGTCGCCGTAGCAAA	CTGTTGCGTATTCAGCTCCT	45.0	50.0	58.0	58.0	1277 bp	0.5	59.8
trnH-GUG-psbA_p3	AAGCTTAACACAAAGGCGGA	CTGTTGCGTATTCAGCTCCT	45.0	50.0	58.0	58.0	1139 bp	0.5	59.7
trnH-GUG-psbA_p4	AGCTTAACACAAAGGCGGAA	CTGTTGCGTATTCAGCTCCT	45.0	50.0	58.0	58.0	1138 bp	0.5	59.7
trnH-GUG-psbA_p5	TGGGTAGAGCCGGATCTAAG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.0	58.0	1271 bp	0.5	59.6