Hotspot

rps16

LSC · 3464-4644 · 1181 bp

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Pi 0.0499

MarkerSeek score 84.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1181
variable_sites: 128
pi: 0.0499
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTCGAGACGCCATTGTCAAT	ACGAGACTTTTTCTACGCCC	45.0	50.0	57.9	57.9	2141 bp	0.2	46.6
rps16_p2	GTTGAGTTAGCAACCCGGAT	ACGAGACTTTTTCTACGCCC	50.0	50.0	57.9	57.9	2487 bp	0.2	46.5
rps16_p3	AAAGCCGAGTACTCTACCGT	ACGAGACTTTTTCTACGCCC	50.0	50.0	58.2	57.9	2505 bp	0.2	46.0
rps16_p4	AAGCCGAGTACTCTACCGTT	ACGAGACTTTTTCTACGCCC	50.0	50.0	58.2	57.9	2504 bp	0.2	46.0
rps16_p5	TTCGAGACGCCATTGTCAAT	CGAGACTTTTTCTACGCCCT	45.0	50.0	57.9	57.6	2140 bp	0.2	44.3