Hotspot

trnL-UAG-rpl32

SSC · 83425-84000 · 576 bp

Back to Neottia

Pi 0.0739

MarkerSeek score 82.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 576
variable_sites: 84
pi: 0.0739
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	GTCCCGAAGAAATCCAACGA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.9	58.0	967-1095 bp	1.0	91.0
trnL-UAG-rpl32_p2	TCCTAAGAGCAGCGTGTCTA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.9	58.0	622-750 bp	1.0	90.7
trnL-UAG-rpl32_p3	TCCTAAGAGCAGCGTGTCTA	CAACATTCTCCATAGGGCCC	50.0	55.0	57.9	58.0	1206-2792 bp	0.4	60.8
trnL-UAG-rpl32_p4	GTCCCGAAGAAATCCAACGA	CAACATTCTCCATAGGGCCC	50.0	55.0	57.9	58.0	1538 bp	0.2	47.2
trnL-UAG-rpl32_p5	GTCCCGAAGAAATCCAACGA	GCTCGGGTTATGGAAGAAGG	50.0	55.0	57.9	58.0	1497 bp	0.2	46.9