Hotspot

ndhG-ccsA

SSC · 82278-82823 · 546 bp

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Pi 0.071

MarkerSeek score 81.70

Primer available yes

Label ndhG-ccsA

Feature Metadata

label_name: ndhG-ccsA
feature_type: igs
region: SSC
length_bp: 546
variable_sites: 84
pi: 0.071
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ccsA_p1	TTGTGGACCATTGGCGATAG	TCGTTGGATTTCTTCGGGAC	50.0	50.0	58.0	57.9	1060 bp	0.2	47.0
ndhG-ccsA_p2	CCTTTCCTGGACCCTCAGTA	TCGTTGGATTTCTTCGGGAC	55.0	50.0	58.0	57.9	1401 bp	0.2	46.9
ndhG-ccsA_p3	TTCCTGGACCCTCAGTAAGG	TCGTTGGATTTCTTCGGGAC	55.0	50.0	58.0	57.9	1398 bp	0.2	46.9
ndhG-ccsA_p4	CGATAGGATCACCTCGCTTG	TCGTTGGATTTCTTCGGGAC	55.0	50.0	57.9	57.9	1046 bp	0.2	46.8
ndhG-ccsA_p5	TTGTGGACCATTGGCGATAG	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	1392 bp	0.2	46.7