Hotspot

accD-psaI

LSC · 33269-33762 · 494 bp

Back to Neottia

Pi 0.0599

MarkerSeek score 82.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 62
pi: 0.0599
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATGCCTACATTGCGTTTGC	CGACACAACGAATCCTTTGC	45.0	50.0	58.0	58.0	1439 bp	0.2	47.6
accD-psaI_p2	TTGCCGAACCTAATGCCTAC	CGACACAACGAATCCTTTGC	50.0	50.0	58.0	58.0	1450 bp	0.2	47.3
accD-psaI_p3	AATGCCTACATTGCGTTTGC	TGTGATTTGTGATTGCCGGA	45.0	45.0	58.0	58.1	769 bp	0.2	47.2
accD-psaI_p4	GTATGTGCTTCTGGAGGAGC	CGACACAACGAATCCTTTGC	55.0	50.0	58.1	58.0	1631 bp	0.2	47.2
accD-psaI_p5	ACCCGTTTGATCGAGTATGC	CGACACAACGAATCCTTTGC	50.0	50.0	58.1	58.0	1679 bp	0.2	47.1