Hotspot

atpB-rbcL

LSC · 29402-30116 · 715 bp

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Pi 0.0718

MarkerSeek score 84.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 105
pi: 0.0718
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGCCCGGTGTAAAAATGACA	GTCCACACAGTTGTCCATGT	45.0	50.0	58.0	58.0	1063-1349 bp	0.8	83.1
atpB-rbcL_p2	TGCCCGGTGTAAAAATGACA	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	1506-1792 bp	0.8	82.0
atpB-rbcL_p3	TGCCCGGTGTAAAAATGACA	AGAAGATTTGGCTGCTACCG	45.0	50.0	58.0	58.0	1037-1283 bp	0.6	74.8
atpB-rbcL_p4	TAGCTCTCCCAACTGGAACA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1709 bp	0.2	47.8
atpB-rbcL_p5	TAGCTCTCCCAACTGGAACA	AGAAGATTTGGCTGCTACCG	50.0	50.0	58.0	58.0	1240 bp	0.2	47.6