Hotspot

trnF-GAA-ndhJ

LSC · 25495-26058 · 564 bp

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Pi 0.0674

MarkerSeek score 81.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 79
pi: 0.0674
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGAAGACTGGGACTCCATTG	50.0	55.0	58.1	58.0	703-1028 bp	0.6	71.6
trnF-GAA-ndhJ_p2	GGGGATATGGCGAAATTGGT	CGAAGACTGGGACTCCATTG	50.0	55.0	58.0	58.0	1411-2232 bp	0.6	71.3
trnF-GAA-ndhJ_p3	GGCGAAATTGGTAGACGCTA	CGAAGACTGGGACTCCATTG	50.0	55.0	58.1	58.0	1403-2224 bp	0.6	70.8
trnF-GAA-ndhJ_p4	AGGGATAGGTGCAGAGACTC	CGAAGACTGGGACTCCATTG	55.0	55.0	57.9	58.0	1237-2064 bp	0.6	70.8
trnF-GAA-ndhJ_p5	TGCAGAGACTCAATGGAAGC	CGAAGACTGGGACTCCATTG	50.0	55.0	57.9	58.0	1228-2055 bp	0.6	70.6