Hotspot

trnD-GUC-trnY-GUA

LSC · 14421-14707 · 287 bp

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Pi 0.0578
MarkerSeek score 81.30
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
287
variable_sites
36
pi
0.0578
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GTGCTCTGACCGATTGAACT CGAGATGTCTACGCTGGTTC 50.0 55.0 57.9 58.2 326-363 bp 1.0 89.9
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT GGGGACGGACTGTAAATTCG 50.0 55.0 57.9 58.4 350-387 bp 1.0 88.2
trnD-GUC-trnY-GUA_p3 GTGCTCTGACCGATTGAACT GGGACGGACTGTAAATTCGT 50.0 50.0 57.9 57.6 349-386 bp 1.0 88.0
trnD-GUC-trnY-GUA_p4 GTGCTCTGACCGATTGAACT CGGACTGTAAATTCGTTGGC 50.0 50.0 57.9 57.5 345-382 bp 1.0 86.9
trnD-GUC-trnY-GUA_p5 GTGCTCTGACCGATTGAACT GGACTGTAAATTCGTTGGCG 50.0 50.0 57.9 57.5 344-381 bp 1.0 86.9