Hotspot

trnC-GCA-trnD-GUC

LSC · 13559-14346 · 788 bp

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Pi 0.0763

MarkerSeek score 78.80

Primer available yes

Label trnC-GCA-trnD-GUC

Feature Metadata

label_name: trnC-GCA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 103
pi: 0.0763
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnD-GUC_p1	ATTTAAATTGGCGGCATGGC	CCATGCATATGGCTGGGATT	45.0	50.0	58.0	58.1	966-2927 bp	1.0	87.0
trnC-GCA-trnD-GUC_p2	AGTTTTTAAGCGACGGGTGT	CCATGCATATGGCTGGGATT	45.0	50.0	58.0	58.1	1129-2878 bp	0.6	69.8
trnC-GCA-trnD-GUC_p3	TTAAGCGACGGGTGTTTTGA	CCATGCATATGGCTGGGATT	45.0	50.0	58.0	58.1	1124-2873 bp	0.6	69.7
trnC-GCA-trnD-GUC_p4	GCGCAACATAGGTTAGGTCA	CCATGCATATGGCTGGGATT	50.0	50.0	58.0	58.1	1450-2848 bp	0.4	61.1
trnC-GCA-trnD-GUC_p5	GCGCAACATAGGTTAGGTCA	AGTTCAATCGGTCAGAGCAC	50.0	50.0	58.0	57.9	1428-2826 bp	0.4	60.9