Hotspot

matK-trnK-UUU

LSC · 1895-2730 · 836 bp

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Pi 0.0724

MarkerSeek score 84.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 122
pi: 0.0724
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	45.0	50.0	58.0	57.9	1204-1349 bp	1.0	90.2
matK-trnK-UUU_p2	TCGCGACGAGAATGGAATTT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.0	58.2	1187-1332 bp	1.0	89.8
matK-trnK-UUU_p3	TCGCGACGAGAATGGAATTT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.0	58.2	1186-1331 bp	1.0	89.8
matK-trnK-UUU_p4	TCGCGACGAGAATGGAATTT	ATTGACAATGGCGTCTCGAA	45.0	45.0	58.0	57.9	1550-1705 bp	0.6	72.7
matK-trnK-UUU_p5	ACTAATCGCGACGAGAATGG	ATTGACAATGGCGTCTCGAA	50.0	45.0	57.9	57.9	1555-1710 bp	0.6	71.7