primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TTAGTTTTATTTGAACTTTGGT	GACTCAGTTCTGCAGTGCAA	22	20	22.7	50.0	50.1	58.4	0.0	20.2	10.292866	62	2767	2808-2934	0.600000	32.2
trnK-UUU_p2	trnK-UUU	2	TTAGTTTTATTTGAACTTTGGT	TTGACTCAGTTCTGCAGTGC	22	20	22.7	50.0	50.1	58.4	0.0	14.7	10.292866	62	2767	2810-2936	0.600000	32.2
trnK-UUU_p3	trnK-UUU	3	TTTAGTTTTATTTGAACTTTGGT	GACTCAGTTCTGCAGTGCAA	23	20	21.7	50.0	51.1	58.4	0.0	20.2	10.323574	62	2767	2809-2935	0.600000	32.2
trnK-UUU_p4	trnK-UUU	4	TTTAGTTTTATTTGAACTTTGGT	TTGACTCAGTTCTGCAGTGC	23	20	21.7	50.0	51.1	58.4	0.0	14.7	10.323574	62	2767	2811-2937	0.600000	32.2
trnK-UUU_p5	trnK-UUU	5	TTTTAGTTTTATTTGAACTTTGGT	GACTCAGTTCTGCAGTGCAA	24	20	20.8	50.0	52.0	58.4	0.0	20.2	10.435294	62	2767	2810-2936	0.600000	32.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	20	20	45.0	50.0	58.0	57.9	3.7	0.0	0.114007	1895	2730	1204-1349	1.000000	90.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCGCGACGAGAATGGAATTT	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	58.0	58.2	3.7	17.1	0.173002	1895	2730	1187-1332	1.000000	89.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCGCGACGAGAATGGAATTT	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	58.0	58.2	3.7	17.1	0.173002	1895	2730	1186-1331	1.000000	89.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCGCGACGAGAATGGAATTT	ATTGACAATGGCGTCTCGAA	20	20	45.0	45.0	58.0	57.9	3.7	0.0	0.097889	1895	2730	1550-1705	0.600000	72.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTAATCGCGACGAGAATGG	ATTGACAATGGCGTCTCGAA	20	20	50.0	45.0	57.9	57.9	22.2	0.0	0.214422	1895	2730	1555-1710	0.600000	71.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GCGCAAAAGATCATAGTCGC	CGCAAAGCAAAGACAACGAA	20	20	50.0	45.0	58.0	58.2	0.3	0.0	0.189244	2768	3463	1224-1380	0.800000	82.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.204673	2768	3463	1151-1348	0.800000	82.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GCGCAAAAGATCATAGTCGC	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.0	58.0	0.3	0.0	0.046833	2768	3463	1218-1374	0.600000	75.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TACCTCATCCAAATGCGCAA	GCAAAGACAACGAATTCCGG	20	20	45.0	50.0	57.9	58.0	17.1	0.0	0.157544	2768	3463	1388-1388	0.200000	46.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TTACCTCATCCAAATGCGCA	GCAAAGACAACGAATTCCGG	20	20	45.0	50.0	57.9	58.0	15.0	0.0	0.157544	2768	3463	1389-1389	0.200000	46.7
rps16_p1	rps16	1	TTCGAGACGCCATTGTCAAT	ACGAGACTTTTTCTACGCCC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.169285	3464	4644	2141-2141	0.200000	46.6
rps16_p2	rps16	2	GTTGAGTTAGCAACCCGGAT	ACGAGACTTTTTCTACGCCC	20	20	50.0	50.0	57.9	57.9	0.7	0.0	0.185404	3464	4644	2487-2487	0.200000	46.5
rps16_p3	rps16	3	AAAGCCGAGTACTCTACCGT	ACGAGACTTTTTCTACGCCC	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.244399	3464	4644	2505-2505	0.200000	46.0
rps16_p4	rps16	4	AAGCCGAGTACTCTACCGTT	ACGAGACTTTTTCTACGCCC	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.244399	3464	4644	2504-2504	0.200000	46.0
rps16_p5	rps16	5	TTCGAGACGCCATTGTCAAT	CGAGACTTTTTCTACGCCCT	20	20	45.0	50.0	57.9	57.6	0.0	0.0	0.458714	3464	4644	2140-2140	0.200000	44.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCGGAATTCGTTGTCTTTGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.112354	4645	5480	1443-1627	1.000000	91.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.139072	4645	5480	933-1129	1.000000	90.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGCTCGAGCTCCATCATATG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.0	58.1	34.5	16.9	0.083461	4645	5480	1094-1282	0.600000	75.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCGGAATTCGTTGTCTTTGC	CCAGGGATAACCGGCATAAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.144044	4645	5480	2009-2117	0.600000	74.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGCTCGAGCTCCATCATATG	CCAGGGATAACCGGCATAAC	20	20	55.0	55.0	58.0	58.1	34.5	0.0	0.115151	4645	5480	1659-1660	0.400000	65.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	TTAGTCCCCTCAGCCATCTC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.2	55.5	0.0	0.0	3.741128	6834	7375	599-862	1.000000	61.3
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	TTTAGTCCCCTCAGCCATCT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.7	55.5	0.0	0.0	3.806276	6834	7375	600-863	1.000000	60.8
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	TTAGTCCCCTCAGCCATCTC	AGAACGAATCACACTTTTACCAC	20	23	55.0	39.1	58.2	57.3	0.0	0.0	3.873844	6834	7375	602-865	1.000000	60.2
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	TTAGTCCCCTCAGCCATCTC	GAACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.2	57.3	0.0	0.0	3.873844	6834	7375	601-864	1.000000	60.2
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	TTTAGTCCCCTCAGCCATCT	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	57.7	57.3	0.0	0.0	3.938992	6834	7375	602-865	1.000000	59.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GCGGTCTCCATAGCAAGAAA	TTATAGGTCCAGGCCCTACG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.029546	12939	13477	1290-1290	0.200000	47.8
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GCGGTCTCCATAGCAAGAAA	TACTTGTTTGAGCCACACCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048516	12939	13477	1345-1345	0.200000	47.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCAACAAGTCTACGCGTGAG	TTATAGGTCCAGGCCCTACG	20	20	50.0	55.0	58.0	58.0	24.6	0.0	0.051653	12939	13477	1355-1355	0.200000	47.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCAACAAGTCTACGCGTGAG	TACTTGTTTGAGCCACACCC	20	20	50.0	50.0	58.0	58.0	24.6	0.0	0.070623	12939	13477	1410-1410	0.200000	47.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCCTTCGGACGTGTCAATC	TTATAGGTCCAGGCCCTACG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.079917	12939	13477	956-956	0.200000	47.4
trnC-GCA-trnD-GUC_p1	trnC-GCA-trnD-GUC	1	ATTTAAATTGGCGGCATGGC	CCATGCATATGGCTGGGATT	20	20	45.0	50.0	58.0	58.1	14.3	0.0	0.114786	13559	14346	966-2927	1.000000	87.0
trnC-GCA-trnD-GUC_p2	trnC-GCA-trnD-GUC	2	AGTTTTTAAGCGACGGGTGT	CCATGCATATGGCTGGGATT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.109310	13559	14346	1129-2878	0.600000	69.8
trnC-GCA-trnD-GUC_p3	trnC-GCA-trnD-GUC	3	TTAAGCGACGGGTGTTTTGA	CCATGCATATGGCTGGGATT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.116150	13559	14346	1124-2873	0.600000	69.7
trnC-GCA-trnD-GUC_p4	trnC-GCA-trnD-GUC	4	GCGCAACATAGGTTAGGTCA	CCATGCATATGGCTGGGATT	20	20	50.0	50.0	58.0	58.1	0.3	0.0	0.085520	13559	14346	1450-2848	0.400000	61.1
trnC-GCA-trnD-GUC_p5	trnC-GCA-trnD-GUC	5	GCGCAACATAGGTTAGGTCA	AGTTCAATCGGTCAGAGCAC	20	20	50.0	50.0	58.0	57.9	0.3	0.0	0.104596	13559	14346	1428-2826	0.400000	60.9
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GTGCTCTGACCGATTGAACT	CGAGATGTCTACGCTGGTTC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.257223	14421	14707	326-363	1.000000	89.9
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GTGCTCTGACCGATTGAACT	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	57.9	58.4	0.0	0.0	0.437550	14421	14707	350-387	1.000000	88.2
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GTGCTCTGACCGATTGAACT	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.461227	14421	14707	349-386	1.000000	88.0
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GTGCTCTGACCGATTGAACT	CGGACTGTAAATTCGTTGGC	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.606741	14421	14707	345-382	1.000000	86.9
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GTGCTCTGACCGATTGAACT	GGACTGTAAATTCGTTGGCG	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.606741	14421	14707	344-381	1.000000	86.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	14924	15969	892-1134	1.000000	90.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	14924	15969	889-1131	1.000000	90.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	14924	15969	870-1112	1.000000	89.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.367761	14924	15969	867-1109	1.000000	89.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	ACCATGGCGTTACTCTACCA	20	20	50.0	50.0	57.8	58.4	0.0	13.0	0.605908	14924	15969	893-1135	1.000000	87.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TAAAACCTGGCCTGTTGACC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.014917	23905	24752	1469-1536	1.000000	91.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TAAAACCTGGCCTGTTGACC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.0	58.1	3.3	20.1	0.062189	23905	24752	2244-2728	1.000000	91.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AGAAAAAGAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.0	0.0	0.063299	23905	24752	871-949	0.800000	83.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGATCCGCAAAGCAAAACTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022012	23905	24752	1189-1224	0.600000	75.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGATCCGCAAAGCAAAACTC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.0	58.1	0.0	20.1	0.069284	23905	24752	1932-2416	0.600000	73.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CGAAGACTGGGACTCCATTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.067632	25495	26058	703-1028	0.600000	71.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGGATATGGCGAAATTGGT	CGAAGACTGGGACTCCATTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.020360	25495	26058	1411-2232	0.600000	71.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGCGAAATTGGTAGACGCTA	CGAAGACTGGGACTCCATTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.085168	25495	26058	1403-2224	0.600000	70.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AGGGATAGGTGCAGAGACTC	CGAAGACTGGGACTCCATTG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.096390	25495	26058	1237-2064	0.600000	70.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGCAGAGACTCAATGGAAGC	CGAAGACTGGGACTCCATTG	20	20	50.0	55.0	57.9	58.0	5.0	0.0	0.120742	25495	26058	1228-2055	0.600000	70.6
atpB-rbcL_p1	atpB-rbcL	1	TGCCCGGTGTAAAAATGACA	GTCCACACAGTTGTCCATGT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.050021	29402	30116	1063-1349	0.800000	83.1
atpB-rbcL_p2	atpB-rbcL	2	TGCCCGGTGTAAAAATGACA	AGAAACGATCTCTCCAACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.019671	29402	30116	1506-1792	0.800000	82.0
atpB-rbcL_p3	atpB-rbcL	3	TGCCCGGTGTAAAAATGACA	AGAAGATTTGGCTGCTACCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.047178	29402	30116	1037-1283	0.600000	74.8
atpB-rbcL_p4	atpB-rbcL	4	TAGCTCTCCCAACTGGAACA	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026507	29402	30116	1709-1709	0.200000	47.8
atpB-rbcL_p5	atpB-rbcL	5	TAGCTCTCCCAACTGGAACA	AGAAGATTTGGCTGCTACCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	29402	30116	1240-1240	0.200000	47.6
accD-psaI_p1	accD-psaI	1	AATGCCTACATTGCGTTTGC	CGACACAACGAATCCTTTGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054612	33269	33762	1439-1439	0.200000	47.6
accD-psaI_p2	accD-psaI	2	TTGCCGAACCTAATGCCTAC	CGACACAACGAATCCTTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.082209	33269	33762	1450-1450	0.200000	47.3
accD-psaI_p3	accD-psaI	3	AATGCCTACATTGCGTTTGC	TGTGATTTGTGATTGCCGGA	20	20	45.0	45.0	58.0	58.1	0.0	5.0	0.102146	33269	33762	769-769	0.200000	47.2
accD-psaI_p4	accD-psaI	4	GTATGTGCTTCTGGAGGAGC	CGACACAACGAATCCTTTGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.102783	33269	33762	1631-1631	0.200000	47.2
accD-psaI_p5	accD-psaI	5	ACCCGTTTGATCGAGTATGC	CGACACAACGAATCCTTTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.113596	33269	33762	1679-1679	0.200000	47.1
clpP-psbB_p1	clpP-psbB	1	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.8	58.2	3.6	0.0	0.405618	42164	42793	672-1047	1.000000	87.7
clpP-psbB_p2	clpP-psbB	2	TTTGGAACACCAATGGGCAT	CGATACCAAGGCAAACCCAT	20	20	45.0	50.0	58.3	58.2	3.6	0.0	0.510230	42164	42793	670-1045	1.000000	86.8
clpP-psbB_p3	clpP-psbB	3	TCCAGGACTCCGAAAAGGTA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.551363	42164	42793	692-1067	1.000000	86.4
clpP-psbB_p4	clpP-psbB	4	CCAGGACTCCGAAAAGGTAC	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.6	58.2	0.0	0.0	0.619017	42164	42793	691-1066	1.000000	85.9
clpP-psbB_p5	clpP-psbB	5	CTTTTGGAACACCAATGGGC	ACACGATACCAAGGCAAACC	20	20	50.0	50.0	57.8	58.5	3.6	0.0	0.638003	42164	42793	675-1050	1.000000	85.8
ndhG-ccsA_p1	ndhG-ccsA	1	TTGTGGACCATTGGCGATAG	TCGTTGGATTTCTTCGGGAC	20	20	50.0	50.0	58.0	57.9	1.5	0.0	0.125907	82278	82823	1060-1060	0.200000	47.0
ndhG-ccsA_p2	ndhG-ccsA	2	CCTTTCCTGGACCCTCAGTA	TCGTTGGATTTCTTCGGGAC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.136328	82278	82823	1401-1401	0.200000	46.9
ndhG-ccsA_p3	ndhG-ccsA	3	TTCCTGGACCCTCAGTAAGG	TCGTTGGATTTCTTCGGGAC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.136328	82278	82823	1398-1398	0.200000	46.9
ndhG-ccsA_p4	ndhG-ccsA	4	CGATAGGATCACCTCGCTTG	TCGTTGGATTTCTTCGGGAC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.146453	82278	82823	1046-1046	0.200000	46.8
ndhG-ccsA_p5	ndhG-ccsA	5	TTGTGGACCATTGGCGATAG	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	1.5	0.0	0.161235	82278	82823	1392-1392	0.200000	46.7
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	GTCCCGAAGAAATCCAACGA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.127156	83425	84000	967-1095	1.000000	91.0
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TCCTAAGAGCAGCGTGTCTA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.162484	83425	84000	622-750	1.000000	90.7
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TCCTAAGAGCAGCGTGTCTA	CAACATTCTCCATAGGGCCC	20	20	50.0	55.0	57.9	58.0	0.0	16.5	0.137395	83425	84000	1206-2792	0.400000	60.8
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	GTCCCGAAGAAATCCAACGA	CAACATTCTCCATAGGGCCC	20	20	50.0	55.0	57.9	58.0	0.0	16.5	0.102067	83425	84000	1538-1538	0.200000	47.2
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GTCCCGAAGAAATCCAACGA	GCTCGGGTTATGGAAGAAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.134023	83425	84000	1497-1497	0.200000	46.9