Hotspot

rpl16

LSC · 80701-82200 · 1500 bp

Back to Neotorularia

Pi 0.0376

MarkerSeek score 66.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 56
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	50.0	50.0	58.0	58.0	2350-2359 bp	1.0	82.2
rpl16_p2	GTCTCAATTCCCGTGGGATC	GTATGGAGGGAATTGCACGT	55.0	50.0	58.0	58.0	2609-2618 bp	1.0	81.9
rpl16_p3	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2381-2390 bp	1.0	81.7
rpl16_p4	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	1998 bp	0.5	58.9
rpl16_p5	GTCTCAATTCCCGTGGGATC	TGCACGTGTTGAATGGATCA	55.0	45.0	58.0	58.1	2257 bp	0.5	58.8