Hotspot

petL-petG

LSC · 65495-65679 · 185 bp

Back to Neotorularia

Pi 0.0919

MarkerSeek score 76.10

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 17
pi: 0.0919
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	GCAGCTTTAACTATAACCTCGG	TGATCACCACGCCTGTATTG	45.5	50.0	56.9	58.0	346-347 bp	1.0	59.3
petL-petG_p2	GCAGCTTTAACTATAACCTCGG	GATCACCACGCCTGTATTGT	45.5	50.0	56.9	58.0	345-346 bp	1.0	59.3
petL-petG_p3	GCAGCTTTAACTATAACCTCGG	AAGTCCAACTGATCACCACG	45.5	50.0	56.9	57.8	355-356 bp	1.0	58.0
petL-petG_p4	AGCAGCTTTAACTATAACCTCGG	TGATCACCACGCCTGTATTG	43.5	50.0	58.3	58.0	347-348 bp	1.0	57.3
petL-petG_p5	AGCAGCTTTAACTATAACCTCGG	GATCACCACGCCTGTATTGT	43.5	50.0	58.3	58.0	346-347 bp	1.0	57.3