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psbE-petL

LSC · 64114-65398 · 1285 bp

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Pi 0.0479

MarkerSeek score 76.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 60
pi: 0.0479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.8	57.5	1339-1358 bp	1.0	45.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACTAGTTATAGTAGGCA	55.0	34.6	58.2	57.5	1334-1353 bp	1.0	45.5
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.4	57.5	1332-1351 bp	1.0	45.5
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.6	57.5	1333-1352 bp	1.0	45.5
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	57.4	57.5	1340-1359 bp	1.0	45.5