Hotspot

trnR-AGA-atpA

LSC · 9373-9679 · 307 bp

Back to Neotorularia

Pi 0.057
MarkerSeek score 75.70
Primer available yes
Label trnR-AGA-atpA

Feature Metadata

label_name
trnR-AGA-atpA
feature_type
igs
region
LSC
length_bp
307
variable_sites
17
pi
0.057
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-AGA-atpA_p1 GGTTCAAATCCTATTGGACGC ACATTAACCGCTGAAGCAGA 47.6 45.0 57.3 57.5 409-413 bp 1.0 67.7
trnR-AGA-atpA_p2 GGTTCAAATCCTATTGGACGC AGACATTAACCGCTGAAGCA 47.6 45.0 57.3 57.5 411-415 bp 1.0 67.7
trnR-AGA-atpA_p3 GGTTCAAATCCTATTGGACGC GACATTAACCGCTGAAGCAG 47.6 50.0 57.3 57.2 410-414 bp 1.0 65.0
trnR-AGA-atpA_p4 GGTTCAAATCCTATTGGACGC TAACCGCTGAAGCAGAAAGC 47.6 50.0 57.3 58.8 405-409 bp 1.0 64.8
trnR-AGA-atpA_p5 GGTTCAAATCCTATTGGACGC ACCAAGACATTAACCGCTGA 47.6 45.0 57.3 57.1 415-419 bp 1.0 64.6