Hotspot

petD

LSC · 74994-76228 · 1235 bp

Back to Neomicrocalamus

Pi 0.00244

MarkerSeek score 47.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1235
variable_sites: 3
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACGAAAGAAAATAAGGCGGA	TGAATGAATCAACCTGAAAAATCC	40.0	33.3	55.4	56.2	1277-1281 bp	1.0	40.4
petD_p2	ACGAAAGAAAATAAGGCGGA	TTGAATGAATCAACCTGAAAAATCC	40.0	32.0	55.4	56.8	1278-1282 bp	1.0	40.4
petD_p3	ACGAAAGAAAATAAGGCGGAT	TGAATGAATCAACCTGAAAAATCC	38.1	33.3	56.0	56.2	1277-1281 bp	1.0	40.4
petD_p4	ACGAAAGAAAATAAGGCGGA	GAATGAATCAACCTGAAAAATCC	40.0	34.8	55.4	54.5	1276-1280 bp	1.0	40.4
petD_p5	ACGAAAGAAAATAAGGCGGATT	TGAATGAATCAACCTGAAAAATCC	36.4	33.3	56.7	56.2	1277-1281 bp	1.0	40.4