Hotspot

infA

LSC · 78351-78674 · 324 bp

Back to Neomicrocalamus

Pi 0.00617
MarkerSeek score 44.80
Primer available yes
Label infA

Feature Metadata

label_name
infA
feature_type
gene
region
LSC
length_bp
324
variable_sites
2
pi
0.00617
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA_p1 CGCAAACCAATACGCACTTT TTAAAGCGTATCAGCCGACC 45.0 50.0 58.0 58.1 1058-1074 bp 1.0 80.0
infA_p2 TCCACTACCAGCACCCTTTA TTAAAGCGTATCAGCCGACC 50.0 50.0 58.0 58.1 1320-1336 bp 1.0 79.9
infA_p3 TTGCAAACCCACTGTACGAA TTAAAGCGTATCAGCCGACC 45.0 50.0 58.0 58.1 1284-1300 bp 1.0 79.9
infA_p4 GTAATGCGGCATCTCTTCCA TTAAAGCGTATCAGCCGACC 50.0 50.0 58.0 58.1 1336-1352 bp 1.0 79.8
infA_p5 TAATGCGGCATCTCTTCCAC TTAAAGCGTATCAGCCGACC 50.0 50.0 58.0 58.1 1335-1351 bp 1.0 79.8