Hotspot

rpl32-trnL-UAG

SSC · 107925-108658 · 734 bp

Back to Neomicrocalamus

Pi 0.00817

MarkerSeek score 63.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 734
variable_sites: 6
pi: 0.00817
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGATCATTTTCCAGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	839-840 bp	1.0	78.4
rpl32-trnL-UAG_p2	AGGGTTTTTCTGGGCAAACA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	802 bp	0.5	58.0
rpl32-trnL-UAG_p3	AGGGTTTTTCTGGGCAAACA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	795 bp	0.5	57.5
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAAACA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.1	58.2	816 bp	0.5	57.5
rpl32-trnL-UAG_p5	AGGGTTTTTCTGGGCAAACA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	800 bp	0.5	57.4