Hotspot

infA-rps8

LSC · 78675-78786 · 112 bp

Back to Neomicrocalamus

Pi 0.0625

MarkerSeek score 82.80

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 7
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	TGACTTTTGCTTCTCTCGGG	TTTTGGGCGGAATGGGAATT	50.0	45.0	57.8	58.3	277 bp	1.0	77.9
infA-rps8_p2	GACTTTTGCTTCTCTCGGGT	TTTTGGGCGGAATGGGAATT	50.0	45.0	57.8	58.3	276 bp	1.0	77.9
infA-rps8_p3	TGACTTTTGCTTCTCTCGGG	GGCGGAATGGGAATTGCTAT	50.0	50.0	57.8	58.4	272 bp	1.0	77.7
infA-rps8_p4	GACTTTTGCTTCTCTCGGGT	GGCGGAATGGGAATTGCTAT	50.0	50.0	57.8	58.4	271 bp	1.0	77.7
infA-rps8_p5	GGGTAGGGCTTCCGTAACTA	TTTTGGGCGGAATGGGAATT	55.0	45.0	58.2	58.3	309 bp	1.0	77.3