Hotspot

trnP-UGG-psaJ

LSC · 66276-66663 · 388 bp

Back to Neomicrocalamus

Pi 0.0155

MarkerSeek score 57.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 6
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	498 bp	1.0	79.3
trnP-UGG-psaJ_p2	CCTCGCTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	55.0	45.0	58.1	57.9	574 bp	1.0	79.0
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	499 bp	1.0	78.8
trnP-UGG-psaJ_p4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	506 bp	1.0	78.7
trnP-UGG-psaJ_p5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	503 bp	1.0	76.9