Hotspot

atpI-atpH

LSC · 33366-34186 · 821 bp

Back to Neomicrocalamus

Pi 0.00611

MarkerSeek score 62.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 5
pi: 0.00611
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	955-957 bp	1.0	74.8
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	888-890 bp	1.0	74.8
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	910-912 bp	1.0	74.7
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	895-897 bp	1.0	74.5
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	45.0	55.0	58.6	58.2	968-970 bp	1.0	74.4