Hotspot

trnG-UCC-trnT-GGU

LSC · 13899-15911 · 2013 bp

Back to Neomicrocalamus

Pi 0.0266
MarkerSeek score 67.40
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
2013
variable_sites
52
pi
0.0266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 38.1 52.4 55.5 58.1 2149-2169 bp 1.0 46.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT GACTTACGTCTTACCATGGCA 38.1 47.6 55.5 57.8 2162-2182 bp 1.0 45.9
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCATGGC 38.1 47.6 55.5 57.8 2163-2183 bp 1.0 45.9
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2152-2172 bp 1.0 45.6
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2151-2171 bp 1.0 45.6