Hotspot

ycf1

SSC · 128460-134027 · 5568 bp

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Pi 0.00121

MarkerSeek score 49.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 19
pi: 0.00121
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATCCACCATTGAACGTGA	GACCGAAAAACTATCCCCCG	45.0	55.0	57.2	58.3	300-303 bp	1.0	71.6
ycf1_p2	TCATCCACCATTGAACGTGA	CGACCGAAAAACTATCCCCC	45.0	55.0	57.2	58.3	301-304 bp	1.0	71.5
ycf1_p3	AGTCATCCACCATTGAACGT	CGACCGAAAAACTATCCCCC	45.0	55.0	57.1	58.3	303-306 bp	1.0	71.5
ycf1_p4	AGTCATCCACCATTGAACGT	GACCGAAAAACTATCCCCCG	45.0	55.0	57.1	58.3	302-305 bp	1.0	71.5
ycf1_p5	GTCATCCACCATTGAACGTG	GACCGAAAAACTATCCCCCG	50.0	55.0	57.1	58.3	301-304 bp	1.0	71.1