Hotspot

rpoC2

LSC · 17557-21714 · 4158 bp

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Pi 0.000722

MarkerSeek score 54.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 9
pi: 0.000722
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGTTACTGCATGAATCGGGA	TCCGATTGTTCTCGAATTGGT	45.0	42.9	56.9	57.7	292 bp	1.0	61.4
rpoC2_p2	TGTTACTGCATGAATCGGGA	CCGATTGTTCTCGAATTGGTC	45.0	47.6	56.9	57.3	291 bp	1.0	58.7
rpoC2_p3	ATGTTACTGCATGAATCGGGA	TCCGATTGTTCTCGAATTGGT	42.9	42.9	57.4	57.7	293 bp	1.0	57.4
rpoC2_p4	TGTTACTGCATGAATCGGGAT	TCCGATTGTTCTCGAATTGGT	42.9	42.9	57.4	57.7	292 bp	1.0	57.4
rpoC2_p5	TGTTACTGCATGAATCGGGA	CCGATTGTTCTCGAATTGGT	45.0	45.0	56.9	56.1	291 bp	1.0	57.2