Hotspot

ndhD

SSC · 120129-121634 · 1506 bp

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Pi 0.000664

MarkerSeek score 42.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 3
pi: 0.000664
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGCATTATACAGTGAACGCT	ACCGACAAAAACCCGTACTC	40.0	50.0	55.2	58.1	1655 bp	1.0	56.5
ndhD_p2	TGCATTATACAGTGAACGCT	GCGGGTTTTTCTGGTCAAAG	40.0	50.0	55.2	57.9	1618 bp	1.0	56.5
ndhD_p3	TGCATTATACAGTGAACGCT	CGACAAAAACCCGTACTCGA	40.0	50.0	55.2	58.2	1653 bp	1.0	55.7
ndhD_p4	TGCATTATACAGTGAACGCTT	ACCGACAAAAACCCGTACTC	38.1	50.0	56.0	58.1	1655 bp	1.0	54.9
ndhD_p5	TTGCATTATACAGTGAACGCT	ACCGACAAAAACCCGTACTC	38.1	50.0	56.0	58.1	1656 bp	1.0	54.9