Hotspot

ccsA-ndhD

SSC · 119892-120128 · 237 bp

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Pi 0.0113

MarkerSeek score 60.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 237
variable_sites: 5
pi: 0.0113
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTGGATTCGGCAATTGTGG	TCCTGATTTCGTTCTCTCGC	52.6	50.0	57.3	57.7	424-428 bp	1.0	65.5
ccsA-ndhD_p2	GTGGATTCGGCAATTGTGG	CCTGATTTCGTTCTCTCGCT	52.6	50.0	57.3	57.7	423-427 bp	1.0	65.5
ccsA-ndhD_p3	TGGATTCGGCAATTGTGGC	TCCTGATTTCGTTCTCTCGC	52.6	50.0	59.1	57.7	423-427 bp	1.0	62.4
ccsA-ndhD_p4	TGGATTCGGCAATTGTGGC	CCTGATTTCGTTCTCTCGCT	52.6	50.0	59.1	57.7	422-426 bp	1.0	62.3
ccsA-ndhD_p5	GGCAATTGTGGCTTCTATAGGA	TCCTGATTTCGTTCTCTCGC	45.5	50.0	58.2	57.7	416-420 bp	1.0	61.9