Hotspot

ndhF-rpl32

SSC · 116107-117242 · 1136 bp

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Pi 0.00123

MarkerSeek score 64.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1136
variable_sites: 4
pi: 0.00123
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	950-1250 bp	1.0	81.7
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	944-1244 bp	1.0	81.6
ndhF-rpl32_p3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	945-1245 bp	1.0	79.8
ndhF-rpl32_p4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1008-1308 bp	1.0	79.7
ndhF-rpl32_p5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1002-1302 bp	1.0	79.6