Hotspot

ycf4-cemA

LSC · 63638-64552 · 915 bp

Back to Neolitsea

Pi 0.00109

MarkerSeek score 59.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 3
pi: 0.00109
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	AGATCCACCAGGGCAAAAAG	55.0	50.0	58.0	58.1	1086-1088 bp	1.0	79.6
ycf4-cemA_p2	CATTCCCTTGACCCGTACTG	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.0	58.1	1087-1089 bp	1.0	79.6
ycf4-cemA_p3	CATTCCCTTGACCCGTACTG	GATCCACCAGGGCAAAAAGA	55.0	50.0	58.0	58.1	1085-1087 bp	1.0	79.6
ycf4-cemA_p4	ATTCCCTTGACCCGTACTGA	AGATCCACCAGGGCAAAAAG	50.0	50.0	58.1	58.1	1085-1087 bp	1.0	79.4
ycf4-cemA_p5	ATTCCCTTGACCCGTACTGA	GATCCACCAGGGCAAAAAGA	50.0	50.0	58.1	58.1	1084-1086 bp	1.0	79.3