Hotspot

trnG-GCC-trnfM-CAU

LSC · 38809-38972 · 164 bp

Back to Neolitsea

Pi 0.00414
MarkerSeek score 60.00
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
164
variable_sites
2
pi
0.00414
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGTGCGGATATGGTCGAATG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 265-272 bp 1.0 81.1
trnG-GCC-trnfM-CAU_p2 AGTGCGGATATGGTCGAATG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 301-308 bp 1.0 80.9
trnG-GCC-trnfM-CAU_p3 GAAAGTGCGGATATGGTCGA CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 268-275 bp 1.0 80.6
trnG-GCC-trnfM-CAU_p4 GAAAGTGCGGATATGGTCGA GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 304-311 bp 1.0 80.4
trnG-GCC-trnfM-CAU_p5 AGAAAGTGCGGATATGGTCG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 305-312 bp 1.0 80.3