Hotspot

psbM-trnD-GUC

LSC · 31387-32361 · 975 bp

Back to Neolitsea

Pi 0.00157

MarkerSeek score 50.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 4
pi: 0.00157
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGAACAACGCAGTGGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.4	57.9	1080-1087 bp	1.0	77.0
psbM-trnD-GUC_p2	TGAACAACGCAGTGGCAATA	ATGCTGTACACCCCATTTCC	45.0	50.0	58.4	57.9	1109-1116 bp	1.0	76.5
psbM-trnD-GUC_p3	GAACAACGCAGTGGCAATAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.3	57.9	1079-1086 bp	1.0	74.6
psbM-trnD-GUC_p4	GAACAACGCAGTGGCAATAA	ATGCTGTACACCCCATTTCC	45.0	50.0	57.3	57.9	1108-1115 bp	1.0	74.1
psbM-trnD-GUC_p5	GAACAACGCAGTGGCAATAAA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	58.0	57.9	1079-1086 bp	1.0	72.1