Hotspot

trnK-UUU-rps16

LSC · 4368-5192 · 825 bp

Back to Neolitsea

Pi 0.00255

MarkerSeek score 66.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 5
pi: 0.00255
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	834-965 bp	1.0	82.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	833-964 bp	1.0	82.9
trnK-UUU-rps16_p3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	45.0	45.0	57.8	58.0	855-986 bp	1.0	82.6
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	843-974 bp	1.0	82.0
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	837-968 bp	1.0	81.2