Hotspot

matK

LSC · 1982-3499 · 1518 bp

Back to Neolamarckia

Pi 0.00725
MarkerSeek score 62.90
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1518
variable_sites
11
pi
0.00725
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACATGCTACATCAACATTTCAGA TCGTCTACCTTTGCTTTGGT 34.8 45.0 56.5 57.1 1655-1658 bp 1.0 40.5
matK_p2 ACATGCTACATCAACATTTCAGA TCGTCTACCTTTGCTTTGGTT 34.8 42.9 56.5 57.8 1655-1658 bp 1.0 40.5
matK_p3 ACATGCTACATCAACATTTCAGAA TCGTCTACCTTTGCTTTGGT 33.3 45.0 57.2 57.1 1655-1658 bp 1.0 40.5
matK_p4 ACATGCTACATCAACATTTCAG TCGTCTACCTTTGCTTTGGT 36.4 45.0 55.1 57.1 1655-1658 bp 1.0 40.5
matK_p5 ACATGCTACATCAACATTTCAGA ATCGTCTACCTTTGCTTTGGT 34.8 42.9 56.5 57.6 1656-1659 bp 1.0 40.5