Hotspot

trnN-GUU-trnR-ACG

IRa · 130894-131457 · 564 bp

Back to Neolamarckia

Pi 0.00887

MarkerSeek score 63.10

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 564
variable_sites: 5
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	656 bp	1.0	77.6
trnN-GUU-trnR-ACG_p2	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	660 bp	1.0	76.7
trnN-GUU-trnR-ACG_p3	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	699 bp	1.0	76.4
trnN-GUU-trnR-ACG_p4	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.7	57.8	648 bp	1.0	76.3
trnN-GUU-trnR-ACG_p5	TTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.3	57.8	657 bp	1.0	73.6